Hydrogen/deuterium Exchange Studies on Copper-zinc Superoxide Dismutase

Hydrogen/deuterium Exchange Studies on Copper-zinc Superoxide Dismutase

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A series of ALS-causing SOD1 mutant proteins were isolated from a yeast expression system and characterized using both global and site-specific HDX. Our HDX studies primarily focused on the apo forms of these proteins, since this form is likely to represent the most aggregation prone state. In the case of apo A4V SOD1, we discovered that this protein features more solvent-accessible domains than human wild-type (hWT) SOD1 does. In addition, apo A4V undergoes a slow local unfolding process that does not occur in hWT. Also, our studies revealed that in its disulfide-reduced form, apo A4V exchanges as if it were a random-coil polypeptide--in contrast, the corresponding form of hWT is a structured protein.... California Institute of Technology Pasadena, CA M.S. in Chemistry University of California at Berkeley Berkeley, CA U.S. Department of Education GAANN Fellowship University of California, Los Angeles Research Mentorship Fellowshipanbsp;...

Title:Hydrogen/deuterium Exchange Studies on Copper-zinc Superoxide Dismutase
Author: Armando Durazo
Publisher:ProQuest - 2007

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